Fig 1: The formation of neurofibrillary tangles (NFTs) in AßO-monkeys(A and B) Immunostaining analysis for detecting NFTs from anterior to posterior brain sections of AßO-monkeys and representative brain regions containing AT8-positive tau tangles in neurons (immunohistochemical images in A and immunofluorescent images in B). The arrows indicate NFTs in soma bodies, and arrowheads indicate long axons with droplet-like staining.(C) The double immunostaining with AT8 and neuronal antibody NF-H.(D) Immunohistochemical analysis for detecting NFTs from anterior to posterior brain sections of AßO-monkeys and representative brain regions containing AT8-positive tau tangles in astrocytes.(E) The double immunostaining with AT8 and astrocyte antibody GFAP. Scale bars: 100 µm (B), 50 µm (A, C, and D), and 25 µm (E). Abbreviations: PFC, prefrontal cortex; PC, parietal cortex; TC, temporal cortex; EC, entorhinal cortex; HPC, hippocampus; MS, medium septum. See also Figures S2 and S3.